Decrease in colocalization of syntrophin and dystrophin on AGE-modified laminin.

Abstract

<p>rMC-1 cells were propagated on AGE-modified laminin followed by staining with syntrophin and dystrophin antibodies, the images were taken using confocal microscopy. (<b>A</b>) Representative photomicrographs showing a decrease in the staining of dystrophin and clustering of syntrophin near cell membrane on AGE-modified laminin. Bar chart showing a Mander’s colocalization coefficient for (<b>B</b>) Syntrophin and (<b>C</b>) Dystrophin, n = 3.</p