Immunofluorescence staining of ferroportin in the duodenum of Alk3fl/fl and Alk3fl/fl; Alb-Cre mice. Ferroportin immunofluorescence staining of formalin fixed paraffin sections of the villosities of the duodenum with 20 times magnification. (Panel a) Control mice with nuclear (DAPI) and ferroportin (FITC) staining of a duodenal section. Cutout images merged (nuclear and ferroportin) and ferroportin (FITC) alone. (Panel b) Duodenal section of hepatocyte-specific Alk3 deficient mice with cutout images merged (nuclear and ferroportin) and ferroportin (FITC) alone. White arrows highlight specific FPN staining. (TIFF 3601 kb