Additional file 4: of Three endoplasmic reticulum-associated fatty acyl-coenzyme a reductases were involved in the production of primary alcohols in hexaploid wheat (Triticum aestivum L.)

Abstract

Figure S4. Genetic transformation of TaFAR6, TaFAR7 and TaFAR8 in tomato cv MicroTom and rice cv Zhonghua 11. a, Schematic representation of constructs used in the transformation experiments. LB, T-DNA left border; 35S polyA, CaMV 35S polyA; Hygromycin, Hygromycin resistance gene; Tnos, NOS terminator; RB, T-DNA right border. b, Plant architecture of T1 transgenic lines at the flowering stage. c, PCR screening of transgenic T1 generation tomato plants by detecting the presence of TaFARs genes. d, Expression analysis of three TaFARs in different overexpression transgenic lines and CK by qRT-PCR. e, Plant architecture of T1 transgenic rice lines at the filling stage. f, PCR screening of transgenic T1 generation rice plants by detecting the presence of TaFARs genes. g, Expression analysis of three TaFARs in different overexpression transgenic rice lines and CK by qRT-PCR. (PDF 10567 kb

    Similar works