Application of expired platelets in the preparation of platelet gel and study of proliferative effects of expired platelet derived growth factor on variety of cell lines
Background and Objectives: There is growing evidence indicating that growth factors derived from platelets can be used in wound healing. This study aimed to investigate whether old platelets can be used as the main
material for preparation o f platelet gel and as substitute for FBS and FCS in cell culture medium.
Materials and Methods: In this exprimental study, platelets were prepared from voluntary blood donors by
centrifugation. To prove the hypothesis that the platelet gel and the growth factor derived from expired platelets are able to pro pagate different cells, platelet derived factors were prepared from both new and expired platelet-rich plasma. The concentration of platelet-derived growth factors was measured by ELISA and cell proliferation was measured by MTT assay.
Results: The results showed the high q uality of platelet gel obtained from old platelets. Our results also
revealed that old platelets released growth factors similar to those released by new platelets.
The growth factors derived from old and new platelets had the same proliferation effects on MSC, CHO, and Fibroblast cell lines.
Conclusions: Old platelets released the same growth factors that new platelets did; this showed that old platelets as valuab le constituents of blood are cost effective to be used
