Gint4.T-MP conjugation.

Abstract

<p>(A) Strategy for the conjugation of Gint4.T aptamer and MP peptide. (B) Analysis of click chemistry reactions between Gint4.T 3’-propargyl adenosine and MP by 12% acrylamide 7 M urea electrophoresis in two different reaction conditions: 1:1,25 (aptamer:peptide) ratio and 1:3 (aptamer:peptide) ratio. The star indicates the Gint4.T/peptide conjugates. (C) Characterization of the aptamer portion by RT-PCR. Gint4.T and Gint4.T-MP were reverse-transcribed, amplified, and loaded on a 3% agarose gel. The star indicates Gint4-T, which is 53 nt. Neg. Ctl RT and Neg. Ctl PCR refers to the mix of reverse-transcription and PCR without template.</p