MOESM2 of Overexpression of Rab11-FIP2 in colorectal cancer cells promotes tumor migration and angiogenesis through increasing secretion of PAI-1

Abstract

Additional file 2: Figure S2. Overexpression of Rab11-FIP2 promoted lung metastasis in HCT116-luc cells. Right panel: Representative BLI (Bioluminescence Imaging) images of mice with lung metastasis. HCT116-luc, (colorectal cancer cells expressing luciferase) were preserved in our laboratory and maintained in DMEM with 10% FBS. We established 116/FIP2-Luc cells which stably overexpressing Rab11-FIP2. 116/FIP2-Luc cells and Control cells (1 × 106) were injected into the tail veins of mice. Eight weeks later, the mice were imaged using IVES CCD imaging system. The bioluminescent signal intensity in the region of interest was quantified as total light emission using Living Image Software (Caliper Lifesciences)

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