Sig. A4Segons consta en: Catálogo de Villancicos y Oratorios en la Biblioteca Nacional Siglos XVIII-XIX, nº 1264, l'autor de la lletra és José Vicente Ortí y MayorTotes les p. orlades. - Reclam

Alejandro Saettone (4927843)

Alyson Burtch (4927837)

Anne-Claude Gingras (154225)

Cristina Thuppu Mudalige (4927834)

Jean-Philippe Lambert (25323)

Jeffrey Fillingham (4926832)

Jyoti Garg (219675)

Marcelo Ponce (4927846)

Ronald Pearlman (4927840)

Syed Nabeel-Shah (4926826)

Epigenetics & Chromatin

arXiv

Additional file 3. Ibd1_Alignments_Cloning

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MOESM3 of The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

Additional file 2. Saf5_Alignments_Cloning

MOESM2 of The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

Abstract Background The chromatin remodelers of the SWI/SNF family are critical transcriptional regulators. Recognition of lysine acetylation through a bromodomain (BRD) component is key to SWI/SNF function; in most eukaryotes, this function is attributed to SNF2/Brg1. Results Using affinity purification coupled to mass spectrometry (AP–MS) we identified members of a SWI/SNF complex (SWI/SNFTt) in Tetrahymena thermophila. SWI/SNFTt is composed of 11 proteins, Snf5Tt, Swi1Tt, Swi3Tt, Snf12Tt, Brg1Tt, two proteins with potential chromatin-interacting domains and four proteins without orthologs to SWI/SNF proteins in yeast or mammals. SWI/SNFTt subunits localize exclusively to the transcriptionally active macronucleus during growth and development, consistent with a role in transcription. While Tetrahymena Brg1 does not contain a BRD, our AP–MS results identified a BRD-containing SWI/SNFTt component, Ibd1 that associates with SWI/SNFTt during growth but not development. AP–MS analysis of epitope-tagged Ibd1 revealed it to be a subunit of several additional protein complexes, including putative SWRTt, and SAGATt complexes as well as a putative H3K4-specific histone methyl transferase complex. Recombinant Ibd1 recognizes acetyl-lysine marks on histones correlated with active transcription. Consistent with our AP–MS and histone array data suggesting a role in regulation of gene expression, ChIP-Seq analysis of Ibd1 indicated that it primarily binds near promoters and within gene bodies of highly expressed genes during growth. Conclusions Our results suggest that through recognizing specific histones marks, Ibd1 targets active chromatin regions of highly expressed genes in Tetrahymena where it subsequently might coordinate the recruitment of several chromatin-remodeling complexes to regulate the transcriptional landscape of vegetatively growing Tetrahymena cells

Alejandro Saettone

Jyoti Garg

Jean-Philippe Lambert

Syed Nabeel-Shah

Marcelo Ponce

Alyson Burtch

Cristina Thuppu Mudalige

Anne-Claude Gingras

Ronald E. Pearlman

Jeffrey Fillingham

Directory of Open Access Journals

The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

MOESM8 of The bromodomain-containing protein Ibd1 links multiple chromatin-related protein complexes to highly expressed genes in Tetrahymena thermophila

Abstract
              
                Background
                The chromatin remodelers of the SWI/SNF family are critical transcriptional regulators. Recognition of lysine acetylation through a bromodomain (BRD) component is key to SWI/SNF function; in most eukaryotes, this function is attributed to SNF2/Brg1.
              
              
                Results
                Using affinity purification coupled to mass spectrometry (AP–MS) we identified members of a SWI/SNF complex (SWI/SNFTt) in Tetrahymena thermophila. SWI/SNFTt is composed of 11 proteins, Snf5Tt, Swi1Tt, Swi3Tt, Snf12Tt, Brg1Tt, two proteins with potential chromatin-interacting domains and four proteins without orthologs to SWI/SNF proteins in yeast or mammals. SWI/SNFTt subunits localize exclusively to the transcriptionally active macronucleus during growth and development, consistent with a role in transcription. While Tetrahymena Brg1 does not contain a BRD, our AP–MS results identified a BRD-containing SWI/SNFTt component, Ibd1 that associates with SWI/SNFTt during growth but not development. AP–MS analysis of epitope-tagged Ibd1 revealed it to be a subunit of several additional protein complexes, including putative SWRTt, and SAGATt complexes as well as a putative H3K4-specific histone methyl transferase complex. Recombinant Ibd1 recognizes acetyl-lysine marks on histones correlated with active transcription. Consistent with our AP–MS and histone array data suggesting a role in regulation of gene expression, ChIP-Seq analysis of Ibd1 indicated that it primarily binds near promoters and within gene bodies of highly expressed genes during growth.
              
              
                Conclusions
                Our results suggest that through recognizing specific histones marks, Ibd1 targets active chromatin regions of highly expressed genes in Tetrahymena where it subsequently might coordinate the recruitment of several chromatin-remodeling complexes to regulate the transcriptional landscape of vegetatively growing Tetrahymena cells

Saettone, Alejandro

Garg, Jyoti

Lambert, Jean-Philippe

Nabeel-Shah, Syed

Ponce, Marcelo

Burtch, Alyson

Thuppu Mudalige, Cristina

Gingras, Anne-Claude

Pearlman, Ronald E

Fillingham, Jeffrey

TSpace Repository

Ortí i Major, Josep Vicenç, 1673-1750

Pradas, José, 1689-1757

Repositori d'Objectes Digitals per a l'Ensenyament la Recerca i la Cultura