<p>A) Confocal images of GV oocyte and 2-cell stage embryo probed with RPS14 antibody (red), DNA stained with DAPI (blue); the gray scale shows separated light channels. Scale bars 20 μm. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. B) Confocal images of GV oocyte and 2-cell stage embryo probed with RPS3 antibody (red), DNA stained with DAPI (blue); the gray scale shows separated light channels. The asterisk indicates cumulus cells. Scale bars 20 μm. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. C) Confocal images of GV oocyte and 2-cell stage embryo probed with RPS6(S235/236) antibody (red), DNA stained with DAPI (blue); the gray scale shows separated light channels. Scale bars 20 μm. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. D) Confocal images of GV oocyte and 2-cell stage embryo probed with RPL7 antibody (red); DNA stained with DAPI (blue); the gray scale shows separated light channels. Scale bars 20 μm. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. E) Confocal images of GV oocyte and 2-cell stage embryo probed with RPL24 antibody (red). DNA stained with DAPI (blue); the gray scale images shows separated light channels. Scale bars 20 μm. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. F) Representative images from WBs for RPS14, RPS3, RPS6 (S235/236), RPL7, RPL24 protein expression in GV oocytes and 2-cell stage embryos, and the loading control (GAPDH); WB was repeated 3 times, with 100 oocytes/embryos per experiment. G) Quantification of expression of RPS14, RPS3, RPS6 (S235/236), RPL7 and RPL24 proteins in the oocyte and embryo. Data were normalized to GAPDH. Data are represented as the mean ± s.d.; the values obtained from oocytes were set as 1. The value bars with <i>ns</i> are not significant, the asterisk denotes statistically significant differences *p<0.05; **p<0.01; ***p<0.001.</p
