Differential effect of MyD88 and TRIF deficiency on <i>Ido</i> expression and Treg induction in PLNs of STZ-treated mice.

Abstract

<p>(A) The mRNA expression of the indicated genes was measured by qRT-PCR in the PLNs of MyD88^CD11c-KO (n = 5), TRIF^CD11c-KO (n = 9) and their respective littermate control <i>Myd88</i>^FL (n = 4) and <i>Trif</i>^FL (n = 8) mice one week after the completion of STZ injections. Results are depicted as fold increase compared to untreated mice (buffer-only) of each genotype. (B) FACS analysis for Tregs (CD4⁺CD25⁺Foxp3⁺) in the PLNs of STZ-treated mice one week after the completion of the STZ injections. Representative plots of gated live, CD4⁺ PLN cells stained with CD25 and intracellular Foxp3. Bar graph shows quantification of CD4⁺CD25⁺Foxp3⁺ cells in STZ-treated <i>Myd88</i>^FL (n = 4), MyD88^CD111c-KO (n = 6), <i>Trif</i>^FL (n = 5) and TRIF^CD11c-KO (n = 6), as well as untreated <i>Myd88</i>^FL (n = 3), MyD88^CD111c-KO (n = 2), <i>Trif</i>^FL (n = 2) and TRIF^CD11c-KO (n = 2) mice.</p