Uji Efek Antibakteri Minyak Atsiri Rimpang Temu Kunci (Boesenbergia Pandurata) Terhadap Pertumbuhan Bakteri Staphylococcus Epidermidis Dan Serratia Marcescens Secara In Vitro
Nosocomial infections are a global health care problems. Two of the bacteria that causes nosocomial infections are Staphylococcus epidermidis and Serratia marcescens. Both of these bacteria cause problems because of resistance to antibiotics. Essential oils contained in rempang temu kunci (Boesenbergia pandurata) are potentially exploited as antibacterial sources. The research to know the antibacterial activity of Boesenbergia pandurata essential oil against Staphylococcus epidermidis and Serratia marcescens in vitro. The research was experimental laboratory which used posttest only with control group design method. The subject of the study was the essential oil of Boesenbergia pandurata. Staphylococcus epidermidis and Serratia marcescens were used as samples. Each bacteria received different treatment with 30%, 50%, 75%, and 90% concentration of Boesenbergia pandurata essential oil, positive control, and negative control using diffusion method. A well was created in the Muller Hinton Agar then spread the growing bacteria which had been standardized with 5.0 Mc Farland, then the well was filled with several concentrate essential oil, sterile distilled water as the negative control, amoxicilin and cloramphenicol as the positive control. Then they were incubated in 37°C for 24 hours and the formed obstruction zone was counted. The essential oil of Boesenbergia pandurata has an effective antibacterial activity against Staphylococcus epidermidis and Serratia marcescens in concentrations of 50%, 75%, and 90% where in the post hoc test compared to negative control the value of p was 0.05, while concentration of 30% is not found any inhibitory power. Boesenbergia pandurata essential oils with concentrations of 50%, 75%, and 90% effectively inhibited the growth of Staphylococcus epidermidis and Serratia marcescens on Muller Hinton media
