Classical simulations of protein flexibility remain computationally
expensive, especially for large proteins. A few years ago, we developed a fast
method for predicting protein structure fluctuations that uses a single protein
model as the input. The method has been made available as the CABS-flex web
server and applied in numerous studies of protein structure-function
relationships. Here, we present a major update of the CABS-flex web server to
version 2.0. The new features include: extension of the method to significantly
larger and multimeric proteins, customizable distance restraints and simulation
parameters, contact maps and a new, enhanced web server interface. CABS-flex
2.0 is freely available at http://biocomp.chem.uw.edu.pl/CABSflex
