Specificity of rhinocerase towards various fluorescent substrates.

Abstract

a<p>The values are mean ± S.D. (<i>n</i> = 3).</p><p>100 µg of rhinocerase were incubated with different concentrations of various fluorescent substrates at 37°C for 7 minutes. The amount of AMC released was measured at an excitation wavelength of 366 nm and an emission wavelength of 460 nm at time intervals up to 7 minutes. 100 µg of <i>B. g. rhinoceros</i> venom were also used at the same experimental conditions for comparison. Kinetic parameters were determined from Lineweaver-Burk plots.</p

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