Fmi, <i>D</i>E-cadherin and Arm show intracellular accumulation in PI3K (III)-knockdown pupal wings.

Abstract

<p>(A) Confocal immunodetection showing XY and XZ sections in the plane of pupal wings at 30–32hr APF. Flamingo [Fmi], <i>D</i>E-cadherin [<i>D</i>E-cad] and Armadillo [Arm] (red) were localized to the ZA in the plasma membrane in wild type, but accumulated to the intracellular puncta in <i>dVps15</i>-knockdown cells. The regions where the GFP-2xFYVE and/or dsRNA for <i>dVps15</i> were expressed by the <i>dpp</i>-Gal4 driver are indicated by double-headed arrows. (B) Fmi mostly accumulated to GFP-LAMP1 positive compartments in <i>dVps15</i>-knockdown pupal wing cells at 30 hr APF while <i>D</i>E-cadherin co-localized only partly. Arrows indicate GFP-LAMP1 positive compartments that harbor Fmi or <i>D</i>E-cadherin. Fmi and <i>D</i>E-cadherin location showed little overlap. The scale bars represent 10 µm.</p

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