<p>(A–E) Horizontal EM images of the pupal wings of the wild type (A and E), or <i>dVps15</i> knockdown (B–D), at 30 h APF. A number of electron-dense compartments appeared in the cytoplasm at the apical side (green parentheses in B). These electron-dense compartments appeared to be MVB-like structures (C and D) that were scarcely observed in the wild type cells (A). (D and E) Immuno-EM images of the pupal wings obtained with a 10 nm gold particle-labeled GFP antibody to detect GFP- LAMP1. Signals of anti-GFP antibody in the wild type (E) and <i>dVps15</i>-knockdown wings (D) are shown, respectively. The scale bars represent 1 µm (A–C), 500 nm (D) and 200 nm (E), respectively.</p
