<p>A: NPY-ir perikarya localized in the arcuate nucleus. Examples of close appositions between NPY-ir fibers (red) and GnRH-GFP perikarya and processes (green) in a maximum intensity projection of the image stack (B) and in single 0.5 µm optical slices (C, D). NPY-ir fibers and GnRH-ir terminals were observed in proximity in the median eminence (E), where the presence of close appositions was confirmed by analysis of single 0.5 µm optical slices (F). Scale bars = 50 µm (A), 10 µm (B–D), 50 µm (E), 5 µm (F).</p
