Generation of a conditional <i>Gata3</i> knockout allele.

Abstract

<p>(A) The conditional <i>Gata3</i> knockout allele was generated by crossing <i>Gata3^ex4GFP</i> mice with <i>FLPe</i> germline deleter mice. The FLPe recombinase removed the <i>Ires-GFP-neo</i> cassette, thereby generating a <i>Gata3</i> allele with <i>loxP</i> sites flanking exon 4 (<i>Gata3^flox</i>). Subsequent crosses of <i>Gata3^flox</i> mice with <i>More-Cre</i> germline deleter mice resulted in <i>Gata3</i>-exon 4 excision and the creation of a null allele (<i>Gata3^D</i>). (B) Representative genotyping by PCR for wild-type (+/+), <i>Gata3^flox/flox</i> (flox/flox) and <i>Gata3^Δ/+</i> (Δ/+) mice prepared from tail DNA.</p