<p>LNC from Mt immunized rats (2.5×10<sup>5</sup> per well) were activated with Mt176-90 for 72 hr in the presence of Anti-p277 or Anti-MBP T-cells (5×10<sup>4</sup> per well). The secretion of IFNγ was determined by ELISA, the results are presented as pg/ml±SEM of triplicate cultures. The differences between the groups were significant (p<0.05) for antigen concentrations higher than 0.1 µg/ml. Three independent experiments produced similar results.</p
