<p>AP-HDL, S-HDL-SAA and HDL were resolved by N-PAGE using 4–20% gradient gels with a continuous buffer system consisting of 0.1 M glycine-Tris-HCl, pH 8.6. Samples (30 µg) were loaded onto the gel and electrophoresis carried out at 4°C for 6 h at 150 volts. Bands were detected by staining with Coomassie Blue R250. A), lane 1, AP-HDL, lane 2, S-HDL-SAA, lane 3, float sample after re-centrifugation of S-HDL-SAA at d = 1.25 g/ml. Molecular weight markers are indicated on the left; B), lane 1, HDL, lane 2, S-HDL, lane 3, delipidated apoA-I.</p
