Experimental design partitions genetic and purely-environmental components of variation.

Abstract

<p>Females heterozygous for <i>P582i</i> (<i>Sam1;Sam2;P582i/Sami</i>; left) were crossed to males from each of 9 highly inbred (95% homozygous) <i>RI</i> line backgrounds (different colors) to create matched “clones” of Hsp90 mutant and control male progeny from the same vial and maternal environments and hybrid for <i>Sam</i> and each <i>RI</i> line background.</p