<div><p>Nuclei were prepared from ES cells transfected with control or p150CAF-1 siRNA vector. Nuclei were digested with increasing amounts of DNase I or MNase. (A) After digestion with the indicated nucleases, total DNA was prepared and run onto an agarose gel which was stained with ethidium bromide to reveal bulk genomic DNA.</p><p>(B) The DNA was blotted onto a nylon membrane, which was then hybridized with the α-<sup>32</sup>P-labeled pSAT major satellite repeat probe [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020181#pgen-0020181-b047" target="_blank">47</a>].</p></div
