<p>(A) The overall distribution of different <i>can1</i> mutation categories is not altered between pre-plating (large) and post-plating (small) <i>can1</i> mutants derived from the <i>msnΔ</i> strain. (B) As in the WT strain, pre-plating (large) but not post-plating (small) <i>can1</i> mutants derived from the <i>msnΔ</i> strain contained transcription-associated mutation (TAM) hotspots <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003680#pgen.1003680-Lippert1" target="_blank">[39]</a>. (C) In contrast to the WT strain where there was a significant shift in the types of deletions observed in the large vs small <i>can1</i> colonies (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003680#pgen-1003680-g002" target="_blank">Figure 2C</a>), there was no such shift in the <i>msnΔ</i> mutant. (D) Also unlike the WT strain, in the <i>msnΔ</i> mutant there was no significant change in the types of base pair substitutions recovered from pre- and post-plating <i>can1</i> mutants.</p
