<p>(A) Gene length. The lengths of TmEs are significantly shorter than those in the PEC dataset and total genes. Many of these short genes may be false essential genes. (B) Position of insertions. Essential genes mistakenly assigned to be non-essential by TM often have insertions in the 25% extreme-ends (5% in 5′ end and 20% in 3′ end). These insertions do not completely disrupt a gene’s function. (C) Number of insertions. 75% of the essential genes mistakenly assigned to be non-essential by TM only have one insertion in them.</p
