Clamping of intracellular calcium ([Ca²⁺]_i) and (pH_i) during OGD.

Abstract

<p><b>A</b>. Addition of 2 mM BAPTA in the pipette solution restricted [Ca²⁺]_i rise during OGD. Rhod-2 dye was used to determine [Ca²⁺]_i in presence of BAPTA. <b>B</b>. 2 mM BAPTA did not affect OGD induced acidosis, whereas 150 mM KPO₄ in the pipette, prevented pH change. <b>C</b>. OGD induced change of pH_i in presence of different concentration of HEPES in the pipette. 80 mM HEPES prevented acidosis completely. pH_i was determined ratiometrically using BCECF. <b>D</b>. 80 mM HEPES in the pipette, did not affect OGD-induced [Ca²⁺]_i rise. 150 mM KPO₄ prevented both calcium rise and acidosis (B). [Ca²⁺]_i was measured with fura-2. Values are the mean±SEM recorded from 5–10 cells.</p