Thermodynamics of binding of cJun analogues to cFos. Columns (from left to right) show i) Tm values from thermal denaturation analysis ii) calculated % helicity for each respective pair calculated from circular dichroism spectra and iii) K<sub>D</sub> values calculated from thermal denaturation data.
<p>The remaining three columns give stoichiometry of binding and thermodynamic data calculated from ITC, with TΔS calculated according to the Gibbs Helmholtz equation.</p>*<p>data calculated using the midpoint of the transition from thermal denaturation profiles (and fit as temperature as a function of lnK<sub>D</sub>, with the fit lnK<sub>D</sub> = aT+C where a is the gradient, T is the temperature in Celsius and C is the intercept) and calculated at 20°C.</p>#<p>Calculated according to TΔS = ΔH−ΔG.</p
