Mutation of H231 and D232 residues in the HD-GYP domain of <i>Xoc</i> RpfG disrupts the PDE activity and regulatory function.

Abstract

<p>(A) Purified RpfG_AA-GYP completely lost its PDE activity detected by colorimetric assays. (B–D) RpfG_AA-GYP lost the ability to restore EPS production (B), biofilm formation (C) and virulence to rice (D) of <i>ΔrpfG</i> to the wild-type level. WT: wild-type, EV: empty vector.</p