dNK cells infiltrate infected placental tissues.

Abstract

<p>(A) Two-color 3D-images of chorionic <i>villi</i> explants organ cultures established from first trimester trophoblast either uninfected (AD169⁻) or HCMV-infected (AD169⁺). Nuclei were stained with dapi (cyan). Infiltrating dNK cells (red). Lower and side panels show orthogonal XZ and YZ slices, respectively. Images are from two-photon Z-stack (total of 200 µm). Scale bars = 100 µm. (B) Two-color IHC of 6-µm-thick sections from paraffin-embedded whole placental biopsies of HCMV⁺ pregnancy termination. HCMV⁺ and HCMV⁻ tissue sections are presented. Representative immunostaining of HCMV-IE (blue, alkaline phosphatase staining) and CD56⁺ NK cells (brown, peroxidase staining) (n = 2).</p