Nanoparticles target lymph node dendritic cells better after i.d. vs. i.m. delivery.

Abstract

<p>(<b>a</b>) Blood concentrations of Dy649-labeled NPs after i.v., i.m. and i.d. administration. (<b>b</b>) Heat maps representing the median percentage of NP⁺ cells for indicated cell populations. Note that maxima vary from 10% in total leukocytes to 100% in monocytes. <i>P</i> values were computed by comparing the adjusted means of each organ between i.d. and i.m. for each cell type with a two-tailed Student's t-test. (<b>c</b>) Importance of route of administration for each cellular subtype. The log-likelihood ratio represents the likelihood of the alternate model, i.e. the model without taking account the route of administration, over the likelihood of the full factorial model. <i>P</i> values were computed using the <i>Chi</i> Square test between the alternate model and the full model for each population. For 144 h, <i>n</i> = 2, for all else, <i>n</i>≥4. Leukocytes: CD45⁺, mature myeloid DCs: CD11c⁺CD11b⁺I/A^b+, cross-presenting DCs: CD11c⁺CD8α⁺ I/A^b+, immature myeloid DCs: CD11c⁺CD11b⁺I/A^b−, immature lymphoid DCs: CD11c⁺CD11b⁻I/A^b−, medullary/red pulp (RP) macrophages (MØ): CD11b⁺F4/80⁺, monocytes: CD11b⁺GR1^midSSC^lowF4/80⁺, granulocytes: CD11b⁺GR1^highSSC^high, T cells: CD3ε⁺, B cells: B220⁺. Draining lymph nodes are indicated by Ax: axillary, Br: brachial, In: inguinal, Po: popliteal; Sp: spleen. *<i>p</i>≤0.05, **<i>p</i><0.01, ***<i>p</i><0.005.</p