<p>(A) Growth curve for a typical microcolony, indicating the sum of all cell lengths within the colony. (B) Mean fluorescence intensity (per unit area) within cells, averaged over a microcolony. (C) Single-cell length over time for three different lineages, representing cases with no growth rate decrease (green), a lag phase (blue) and a longer lag phase (red). Arrows indicate cell division events. The curves are vertically shifted for clarity. (D) Elongation rates obtained by exponential fits to the length data at sub-cell cycle resolution. Drawn lines are fitted parameterized functions. ΔTµ<sub>2</sub> is the time difference between the time of shift and the half maximum to growth recovery after shift. (E) Fluorescence levels for the three lineages in (C) and (D). Drawn lines are fitted parameterized functions. ΔT<sub>F</sub> is the time difference between the time of shift and the half maximum to induction after shift. Black bar: 120 min before the shift, over which data was averaged to determine the expression level prior to the shift.</p
