<p>iDCs were generated from adherent PBMCs depleted of T cells. The adherent cells were cultured in serum-free AIM-V medium with GM-CSF and IL-4, harvested on day 5 and incubated with soluble PADRE, particle-bound PADRE or an inflammatory cytokine cocktail of IL-1β, IL-6 and TNF-α for 2 additional days. On day 7 the cells were labelled with antibodies for the HLA-DR, CD11c and Lin (cocktail of FITC-conjugated antibodies against the molecules CD3, CD14, CD19 and CD56) for exclusion, and CD80, CD83 and CD86, and analyzed by flow cytometry. The graphs show mean fold changes with standard error of the expression of CD80 (<b>A</b>), CD86 (<b>B</b>), CD83 (<b>C</b>), and HLA-DR (<b>D</b>). n = 5; NT: no treatment; Cocktail: IL-1β, IL-6 and TNF-α. * = p<0.05; ** = p<0.01; and *** = p<0.001 comparing the group treated with the indicated compound vs. the group “NT”.</p
