<p>iDCs as in the experiments shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063039#pone-0063039-g001" target="_blank">Figure 1</a> were incubated either without stimuli (negative control) or with an inflammatory cytokine cocktail of IL-1β, IL-6 and TNF-α as positive controls, or with particles without cargo or loaded with GILGFVFTL peptide, NLVPMVATV peptide, PADRE, diacyl-lipopeptide P2C, a mix of both PADRE and P2C or with a mixture of particles carrying either PADRE or P2C. After two days cultures the cells were labelled with antibodies for the CD11c and Lin (cocktail of FITC-conjugated antibodies against the molecules CD3, CD14, CD19 and CD56) for exclusion, and CD80, CD83, CD86 and HLA-DR, and analyzed by flow cytometry. The graphs show fold of expression of the molecules CD80 (<b>A</b>), CD86 (<b>B</b>), CD83 (<b>C</b>) and HLA-DR (<b>D</b>) with standard error. n = 6. * = p<0.05; ** = p<0.01; and *** = p<0.001 comparing the group treated with the indicated compound vs. the group “No Treatment”.</p
