Chlorine Dioxide Inactivation of Enterovirus 71 in
Water and Its Impact on Genomic Targets
- Publication date
- Publisher
Abstract
To
control the waterborne transmission of enterovirus 71(EV71),
which is associated with hand foot and mouth disease (HFMD), it is
essential to know the inactivation effectiveness of disinfectants
on EV71 in water. In this article, we present a comparative analysis
of the effects on EV71 following exposure to chlorine dioxide (ClO<sub>2</sub>) under different doses, pH, and temperature conditions. We
show that the EV71 exhibited strong resistance to ClO<sub>2</sub> (more
than the MS2 standard) and that <i>C<sub>t</sub></i> value
ranges required for a 4-log reduction of EV71 in buffered, disinfectant
demand-free water at pH 7.2 and 20 °C by ClO<sub>2</sub> were
4.24–6.62 mg/L·min according to the efficiency factor
Hom model. ClO<sub>2</sub> inactivation of the virus was temperature-
and pH-dependent. The virucidal efficiency was higher at pH 8.2 than
at pH 5.6 and pH 7.2 and higher at 36 °C than at 4 and 20 °C.
In addition, we also observed the impact of ClO<sub>2</sub> on the
entire viral genome using RT-PCR, which indicated that the 5′
noncoding region (5′-NCR) within the EV71 genome, specifically
the 1–118 nt region, was the most easily damaged by ClO<sub>2</sub> and correlated with viral infectivity. Our study has not
only provided guidelines for EV71 disinfection strategies of waste
and drinking water, but also confirmed the importance of the 5′-NCR
for EV71 infectivity and may demonstrate a general inactivation by
ClO<sub>2</sub> of enteric virus by damaging the 5′-NCR. Furthermore,
5′-NCR can be used as a target region for PCR to investigate
infectious virus contamination in environmental water and evaluate
the inactivation effects of ClO<sub>2</sub>