Lanostane Triterpenoids from the Mushroom <i>Naematoloma fasciculare</i>

Abstract

In our continuing search for structurally interesting and bioactive metabolites from Korean wild mushrooms, bioassay-guided fractionation and a chemical investigation of the MeOH extracts of the fruiting bodies of the mushroom <i>Naematoloma fasciculare</i> resulted in the isolation of four new lanostane triterpenoids (<b>1</b>–<b>4</b>), together with 11 known compounds (<b>5</b>–<b>15</b>). The structures of <b>1</b>–<b>5</b> were determined by a combination of 1D and 2D NMR and HRMS. The absolute configuration of the 3-hydroxy-3-methylglutaryl group as a side chain in <b>1</b> and <b>2</b> was determined by the alkaline methanolysis method. The full NMR data assignment of the known compound fasciculol G (<b>5</b>) is reported for the first time. Compounds <b>1</b>–<b>15</b> were tested for their antiproliferative activities against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15) and evaluated for their inhibitory effects on nitric oxide production in a lipopolysaccharide-activated murine microglial cell line