Efficient and Treg-specific deletion of <i>Dgcr8</i> and miR-150.

Abstract

<p>Conventional CD4⁺GFP⁻ (Tc) and CD4⁺GFP⁺ regulatory (Tr) T cells were FACS sorted from FoxP3-GFP reporter mice (a) and FoxP3-GFP-hCre:DGCR8^wt/lox (het) or FoxP3-GFP-hCre:DGCR8^lox/lox (KO) mice (b-d). qPCR analysis of Dgcr8 mRNA in Tc and Tr (a,b) and miR-150 (c,d). Pooled data from 3 independent experiments with 3 mice total (a,b) and representative data from 2 independent experiments with 2 mice total (c,d).</p