Livers from LD fed B6 mice display activated stellate cells and fibrotic lesions.

Abstract

<p>(A) Liver tissue sections were stained with α-smooth muscle actin antibody (αSMA) and cell nuclei were stained with DAPI. α-smooth muscle actin signal (green fluorescence) was normalized to cell number (blue fluorescence) (n = 4 mice per group, 5 tissue sections per mouse; *<i>P</i><0.05 compared to <i>Ccr2^−/−</i> mice; **P<0.05 compared to all groups). A representative section from LD fed B6 mice (B), <i>Ccr2^−/−</i> mice (C) and <i>Cd44^−/−</i> mice (D). (E–H) Low and (J–L) high power magnification of pico-sirius stained SD fed B6 mice (E), LD fed B6 mice (F,J), <i>Ccr2^−/−</i> mice (G,K) and <i>Cd44^−/−</i> mice (H,L). (I) Fibrosis score was determined by sirius-red positive staining (**P<0.05 compared to all groups, *P<0.05 compared to B6 SD and <i>Ccr2^−/−</i> LD). In E–H and J–L the bar corresponds to 25µm.</p