Magnetic Porous Sugar-Functionalized PEG Microgels
for Efficient Isolation and Removal of Bacteria from Solution
- Publication date
- Publisher
Abstract
Here,
we present a new microparticle system for the selective detection
and magnetic removal of bacteria from contaminated solutions. The
novelty of this system lies in the combination of a biocompatible
scaffold reducing unspecific interactions with high capacity for bacteria
binding. We apply highly porous poly(ethylene glycol) (PEG) microparticles
and functionalize them, introducing both sugar ligands for specific
bacteria targeting and cationic moieties for electrostatic loading
of superparamagnetic iron oxide nanoparticles. The resulting magnetic,
porous, sugar-functionalized (MaPoS) PEG microgels are able to selectively
bind and discriminate between different strains of bacteria Escherichia coli. Furthermore, they allow for a highly
efficient removal of bacteria from solution as their increased surface
area can bind three times more bacteria than nonporous particles.
All in all, MaPoS particles represent a novel generation of magnetic
beads introducing for the first time a porous, biocompatible and easy
to functionalize scaffold and show great potential for various biotechnological
applications