<p>Primers flanking the retained intron were used for amplification. <i>RD29A</i> (<i>AT5G52310</i>), a gene induced by cold, was used for verification of the effectiveness of the cold-treatment. Amplification of <i>Cyclophilin</i> (<i>AT4G38740</i>) was used to demonstrate an equal quantity of template in each PCR reaction. Asterisks indicate cold regulated splice variants. Crosses indicate genes with transcripts predicted to trigger NMD (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066511#pone.0066511.s003" target="_blank">Figure S3</a>).</p
