<p>The distribution of HPV16 3′-breakpoints of viral-cellular DNA junctions (n = 74) was analyzed within the five different segments E1, E2, E5, E1-Ac and Ac-PAE of the HPV16 early region. The positions of each segment in the HPV16 genome are given in parentheses. The E1-PAE segment of the HPV16 early region (pos. 865–4215, 3351 bp) was taken as reference. The relative length of each segment is shown by the white bars. The relative frequency of HPV16 3′-breakpoints within each segment is shown by the grey bars for all DNA junctions (DJ_all, n = 74, middle-grey bar), the transcribed DNA junctions (DJ_tr., n = 21, light-grey bar) and the non-transcribed DNA junctions (DJ_n.tr., n = 53, dark-grey bar). The exact two-tailed one-sample binomial test was used for statistical analysis by comparing the relative frequency of HPV16 3′-breakpoints in each segment to the relative segment length. Bars marked with asterisks indicate statistically significant results (P<0.05). Data are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066693#pone.0066693.s005" target="_blank">Table S4</a>.</p
