Description of 122 shared-types (SITs; n = 767 isolates) and corresponding spoligotyping defined lineages/sublineages starting from a total of 794 <i>M. tuberculosis</i> strains isolated from adults with pulmonary tuberculosis in Lima, Peru.

Abstract

*<p>A total of 100/122 SITs (n = 718) matched a preexisting shared-type in the database, whereas 22/122 SITs (n = 49 isolates) were newly created either within the present study or after a match with an orphan in the database. A total of 66 SITs containing 711 isolates were clustered within this study (2 to 130 isolates per cluster), while 56 SITs contained a unique strain within this study.</p><p>Note that SITs followed by an asterisk indicates “newly created shared-type” (n = 22 containing 49 isolates) due to 2 or more strains belonging to an identical new pattern within this study or after a match with an orphan in the database. SIT designations followed by number of strains: 2961* this study (n = 2); 3000* this study (n = 3); 3001* this study (n = 8) and USA (n = 1); 3004* this study (n = 2); 3005* this study (n = 2); 3006* this study (n = 2) and South Africa (n = 1); 3007* this study (n = 1) and USA (n = 1); 3008* this study (n = 1) and USA (n = 1); 3009* this study (n = 2); 3010* this study (n = 1) and USA (n = 1); 3011* this study (n = 4); 3012* this study (n = 2); 3013* this study (n = 3) and USA (n = 1); 3014* this study (n = 1) and Argentina (n = 1); 3015* this study (n = 2); 3016* this study (n = 1) and Panama (n = 1); 3017* this study (n = 3); 3089* this study (n = 2) and Mexico (n = 1); 3168* this study n = 1, Sweden (n = 1); 3431* this study (n = 2); 3432* this study (n = 2); 3433* this study (n = 2), BRA (n = 1).</p>**<p>Lineage designations according to SITVIT2 using revised SpolDB4 rules; “Unknown” designates patterns with signatures that do not belong to any of the major clades described in the database.</p>***<p>Clustered strains correspond to a similar spoligotype pattern shared by 2 or more strains “within this study”; as opposed to unique strains harboring a spoligotype pattern that does not match with another strain from this study. Unique strains matching a preexisting pattern in the SITVIT2 database are classified as SITs, whereas in case of no match, they are designated as “orphan” (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065873#pone-0065873-t001" target="_blank">Table 1</a>).</p

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