Biosynthesis and fates of S1P in U87-MG and glioma stem cells.
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Abstract
<p>U87-MG, U–SC and L0627 cells were pulsed with [<sup>3</sup>H]-sphingosine (Sph) for the indicated periods of time. At the end, cells and media were processed and analyzed as described in “Materials and methods”. Panel A, total incorporated radioactivity and intracellular Sph. Panel B, radioactivity associated to total Sph phosphorylation, total S1P and S1P degradation (<sup>3</sup>H <sub>2</sub>O). Panel C, radioactivity incorporated into intracellular and extracellular S1P. Data are the mean ± SD of at least three independent experiments.</p
