Effect of maternal HFD and Fat-1 transgene on WT adult male offspring weight gain and body composition.

Abstract

<p>(A) WT male offspring weight gain. Offspring from each maternal group (<i>n</i> = 10 mothers per group) were weaned onto CD, and weighed every other week for 16 weeks. (B) Adult male offspring body composition (fat and lean mass) at 16 wks post-wean, as determined by Echo MRI. (C and D) Adult male offspring liver TG deposition. TG were quantitated in (C) by colorimetric assay on tissue lipid extracts, and visualized in (D) by Oil Red O staining of representative liver cryosections for neutral lipids (lipid droplet = red, cell nuclei = blue, 20X magnification). All offspring measures are averaged per mother, with siblings treated as replicates. For Fat-1 mothers, only WT offspring were included in analyses. All data are expressed as mean ± SEM; *<i>P</i><0.05.</p

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