Adaptive trajectories towards the three inverse LacI variants.
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Abstract
<p>The three inverse LacI variants all contain three mutations. Each mutation is represented by a vector (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003580#pgen-1003580-g002" target="_blank">Figure 2</a>). The axes indicate expression without IPTG in Env<sub>0</sub> and expression with IPTG in Env<sub>1</sub>. Expression levels in both environments are normalized to the LacI<sub>wt</sub> level. Note that expression along the vertical axis is represented as (Expression)<sup>−1</sup>, as during inversion the expression level in Env<sub>1</sub> decreases. The inverse, triple mutant, is located in the upper right corner of the plot. A) LacI<sub>inv1</sub>: S97P (blue), R207L (green), T258A (red). B) LacI<sub>inv2</sub>: S97P (blue), L307H (green), L349P (red). C) LacI<sub>inv3</sub>: S97P (blue), G315D (green), P339H (red). The significance of the phenotypic effect of mutations is tested with a <i>t</i>-test with Bonferroni correction for multiple comparisons (<i>P</i><0.05), error-bars are standard deviations, n = 3.</p
