<p>(<i>A</i>) Motif discovery of transcription-factor-binding motifs by the DREME algorithm using all the peaks of the ChIP-seq data for WT or DKO Flk1(+) cells in which Gata4GR was activated by Dex addition. Logos for the most enriched motif identified by DREME and its reverse complement sequence, motif IDs, and E-values are shown. (<i>B,C</i>) Gata4 ChIP-seq enrichment at the Gata4-response gene (<i>B</i>) <i>Aqp8</i> and (<i>C</i>) <i>Sox7</i> loci in WT or DKO Flk1(+) cells in which Gata4GR was activated by Dex, and the DNA methylation state at the transcription-start sites and Gata4-binding sites. Tracks represent the mapped read enrichment as determined by DNAnexus software. Blue arrowheads mark Gata4 peaks enriched in DKO Flk1(+) cells compared to WT Flk1(+) cells. Above the peak profiles, the nucleotide positions and Refseq genes are indicated. Horizontal bars represent the genomic regions subjected to DNA methylation analysis by bisulfite sequencing. Open circles represent unmethylated CpGs, and filled circles represent methylated CpGs. The percentage of total CpGs that were methylated is shown below each bisulfite sequencing profile. “WT”, WT Flk1(+) cells; “DKO”, DKO Flk1(+) cells; “ES”, WT ES cells.</p
