Construction strategy of the recombinant <i>S.</i><i>cerevisiae</i> strain to achieve the co-production of ethanol and xylitol from ligonocellulosic biomass.
<p>The uptake and hydrolysis of cellobiose (derived from cellulose) were accomplished by a cellodextrin transporter (encoded by <i>cdt-1</i>) and an intracellular β-glucosidase (encoded by <i>gh1-1</i>), respectively. The uptake and conversion of xylose (derived from hemicellulose) was accomplished by endogenous hexose transporters and a xylose reductase (encoded by <i>XYL1</i>), respectively. Thus, ethanol and xylitol were produced simultaneously by this engineered yeast. NAD(P)H for xylitol production was provided by the cellobiose metabolism.</p
