Effect of hypotonic stress and elevated K⁺ on the ECS diffusion parameters in α-syn +/+ and α-syn −/− mice <i>in vitro</i>.

Abstract

<p>The values are presented as mean ± S.E.M. Asterisks (*-p<0.05; **-p<0.01;***-p<0.001) indicate significant differences between the values in α-syn +/+ and α-syn −/− animals; crosshatches (#-p<0.05; ##-p<0.01; ###-p<0.001) indicate significant differences between control values and those obtained under experimental conditions in the same group of animals. The control values of the ECS diffusion parameters from each individual experiment were calculated as the average values extracted from three diffusion curves before application. The mean control value presented in the table is the average of the control values from all in vitro experiments. The mean values of the maximum change during application correspond to the time-point of the maximum decrease of α (i.e., the 25^th min in H-50 and H-100 and the 30^th min in 10 mM K⁺); the values for washout correspond to the data point at the 90^th min. Abbreviations: extracellular space volume fraction (α), tortuosity (λ), non-specific uptake (k<i>′</i>), number of animals (N), number of slices (n), hypotonic solutions (H-50 and H-100).</p