Hydrophobized Thermoresponsive Copolymer Brushes for
Cell Separation by Multistep Temperature Change
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Abstract
For preparing a thermally modulated
biointerface that separates
cells without the modification of cell surfaces for regenerative medicine
and tissue engineering, poly(<i>N</i>-isopropylacrylamide-<i>co</i>-butyl methacrylate) (P(IPAAm-<i>co</i>-BMA),
thermo-responsive hydrophobic copolymer brushes with various BMA composition
were formed on glass substrate through a surface-initiated atom transfer
radical polymerization (ATRP). Characterization of the prepared surface
was performed by X-ray photoelectron spectroscopy (XPS), attenuated
total reflection Fourier transform infrared spectroscopy (ATR/FT-IR),
and gel-permeation chromatography (GPC) measurement. Prepared copolymer
brush surfaces were characterized by observing the adhesion (37 °C)
and detachment (20 or 10 °C) of four types of human cells: human
umbilical vein endothelial cells (HUVECs), normal human dermal fibroblasts
(NHDFs), human aortic smooth muscle cells (SMCs), and human skeletal
muscle myoblast cells (HSMMs). HUVECs and NHDFs exhibited their effective
detachment temperature at 20 and 10 °C, respectively. Using cells’
intrinsic temperature sensitivity for detachment from the copolymer
brush, a mixture of green fluorescent protein (GFP)-expressing HUVECs
(GFP-HUVECs) and NHDFs was separated