A Novel Approach of Periodate Oxidation Coupled with
HPLC-FLD for the Quantitative Determination of 3‑Chloro-1,2-propanediol
in Water and Vegetable Oil
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Abstract
A novel approach of periodate oxidation
coupled with high-performance
liquid chromatography (HPLC)–fluorescence detection (FLD) for
the quantitative determination of 3-chloro-1,2-propanediol (3-MCPD)
has been established. The essence of this approach lies in the production
of chloroacetaldehyde by the oxidization cleavage of 3-MCPD with sodium
periodate and the HPLC analysis of chloroacetaldehyde monitored by
an FLD detector after fluorescence derivatization with adenine. The
experimental parameters relating to the efficiency of the derivative
reaction such as concentration of adenine, chloroacetaldehyde reaction
temperature, and time were studied. Under the optimized conditions,
the proposed method can provide high sensitivity, good linearity (<i>r</i><sup>2</sup> = 0.999), and repeatability (percent relative
standard deviations between 2.57% and 3.44%), the limits of detection
and quantification were 0.36 and 1.20 ng/mL, respectively, and the
recoveries obtained for water samples were in the range 93.39–97.39%.
This method has been successfully applied to the analysis of real
water samples. Also this method has been successfully used for the
analysis of vegetable oil samples after pretreatment with liquid–liquid
extraction; the recoveries obtained by a spiking experiment with soybean
oil ranged from 96.27% to 102.42%. In comparison with gas chromatography
or gas chromatography–mass spectrometry, the proposed method
can provide the advantages of simple instrumental requirement, easy
operation, low cost, and high efficiency, thus making this approach
another good choice for the sensitive determination of 3-MCPD