<p>(<b>A, B</b>) Embryonic (stage 17) and larval (L1) expression pattern of the <i>prc</i>>GFP reporter. (<b>C, D</b>) Prc protein becomes trapped in adipocytes of <i>prc</i>><i>sar1</i>-IR knock down first instar larvae, proving that the protein becomes expressed by the fat body. Scale bar is 10 µm. (<b>E</b>) Immunoblots of whole larval extracts probed against Prc. While <i>hand</i>C-Gal4 driven knock down does not alter the total amount of Prc the protein is nearly undetectable in <i>prc</i>-Gal4 driven knock down animals. Total protein was stained with amido black 10B. (<b>F</b>) Percentage of third instar larvae showing pericardial cell detachment induced by <i>prc</i> knock down either driven by <i>hand</i>C-Gal4 or <i>prc</i>-Gal4. <i>n</i> indicates the total number of tested animals. (<b>G</b>) Scheme of Prc matrix formation. Secreted Prc from different sources (heart cells in the embryo, adipocytes in the larva) becomes incorporated into the cardiac ECM dependent on Loh.</p
