<p>Note: Lethal phase is defined as the maximum stage the animals can reach. Some animals die at earlier stages, and if the culture is too crowded, the lethal phase can be earlier. The lethal phases of <i>flw<sup>6</sup></i>, <i>flw<sup>7</sup></i>, and <i>flw<sup>FP41</sup></i> are listed as already published <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070502#pone.0070502-Raghavan1" target="_blank">[23]</a>-<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070502#pone.0070502-Sun1" target="_blank">[25]</a>. In addition, since the eggs for lethal phase analysis were from heterozygous females, maternal contributions were included. Maternal contributions cannot be avoided because Flw is required for oogenesis. All alleles except <i>XE55C</i> were rescued to fertile adults with an <i>flw</i>-carrying duplication <i>Dp(1;3)DC224</i>.</p>*<p>: <i>XE55C</i> itself was not rescued, but <i>XE55C</i> females transheterozygous with <i>XE55D</i> or <i>XE55E</i> were rescued by <i>Dp(1;3)DC224</i> to fertile adults. This suggests that there is a second non-<i>flw</i> lethal hit on the <i>XE55C</i>-carrying chromosome.</p><p>For rescues with Pp1ß9C (<i>flw</i> cDNA under the control of UASt promoter, which expresses Flw-pB in somatic cells specified by Gal4 lines used <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070502#pone.0070502-Raghavan1" target="_blank">[23]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070502#pone.0070502-Vereshchagina1" target="_blank">[24]</a>), the following Gal4 lines were used: tub-Gal4 (<i>w; UAS-Pp1ß9C/CyO; TubGal4/TM6C</i>) expressing Gal4 under the control of the <i>α-tubulin84B</i> promoter, maternal Gal4 (<i>w*; P{w[+mC] = matalpha4-GAL-VP16}V37</i>) expressing GAL4-VP16 fusion protein under the control of the <i>α-tubulin67C</i> promoter, act Gal4-II (<i>y<sup>1</sup>w*; P{w[+mC] = Act5C-GAL4}25FO1/CyO, y<sup>+</sup></i>) and act Gal4-III (<i>y<sup>1</sup>w*; P{w[+mC] = Act5C-GAL4}25FO1/CyO, y<sup>+</sup></i>) expressing Gal4 under the control of <i>actin5C</i> promoter. For rescuing with tubGal4, males of <i>w; UAS-Pp1ß9C/CyO; TubGal4/TM6C</i> were crossed with females of <i>flw<sup>mutations</sup>/FM7</i>. For other Gal4-UASt-Pp1ß9C mediated rescues, females of <i>flw<sup>mutations</sup>/FM7; UASPp1ß9C/CyO</i> were crossed with males of corresponding Gal4 lines. For rescuing with duplication, males carrying <i>Dp(1;3)DC224</i> were crossed with females of <i>flw<sup>mutations</sup>/FM7</i>. For female rescues, rescued <i>XE55</i> mutant males were crossed with females of <i>flw<sup>mutations</sup>/FM7.</i> NA: not available.</p
