ZnO:Er,Yb,Gd
Particles Designed for Magnetic-Fluorescent
Imaging and Near-Infrared Light Triggered Photodynamic Therapy
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Abstract
In
this paper, fluorescent and magnetic bifunctional ZnO:Er,Yb,Gd
particles were synthesized via a simple homogeneous precipitation
method. The morphology, size, fluorescent properties, and magnetic
properties of the particles can be readily modified by doping with
Er<sup>3+</sup>, Yb<sup>3+</sup>, and Gd<sup>3+</sup>. The results
revealed that the ZnO:Er,Yb,Gd particles have both down-conversion
and up-conversion fluorescence after calcination at high temperatures
(>700 °C). The products successfully labled the human hepatocellular
carcinoma (HepG2) cells and presented low toxicity even at a high
concentration of 2 mg/mL. Being upconverting nanoparticles (UCNPs),
the prepared ZnO:Er,Yb,Gd particles exposed to 980 nm near-infrared
(NIR) laser light emitted up-conversion fluorescence which could be
absorbed by a photodynamic therapy (PDT) drug, methylene blue (MB),
and then killed the HepG2 cells via PDT mechanism. In vitro therapeutic
investigation evidenced the prominent PDT effects of MB-loaded ZnO:Er,Yb,Gd
UCNPs upon NIR light irradiation. In magnetic resonance imaging (MRI)
studies, ZnO:Er,Yb,Gd particles revealed a tunable longitudinal relaxivity
rate (<i>r</i><sub>1</sub>) from 23.03 mM<sup>–1 </sup>s<sup>–1</sup> to 36.84 mM<sup>–1</sup> s<sup>–1</sup>, which is much larger than the conventional Gd-DTPA and currently
reported Gd-base nanoparticles, suggesting it would be a good candidate
as an MRI agent. It is expected that these particles have applications
in magnetic-fluorescent bimodal imaging and NIR light triggered photodynamic
therapy