Adenoviral delivery of Ad-Ang-2 rescues the loss of histamine response in Ang-2^−/− mice.

Abstract

<p>WT and Ang-2^−/− mice received an intravenous injection of 10⁹ PFU Ad-LacZ or Ad-Ang-2, respectively. Ang-2 in the serum was semi-quantitatively determined by Western blot after 3 days showing a myc-tagged band of approx. 60 kDa in Ad-Ang-2 treated mice alone (<b>A</b>). Histamine (62.5 µg/g) or saline as negative control were injected together with 100 nm green fluorescent microspheres in either Ad-LacZ or Ad-Ang-2 treated WT and Ang-2^−/− mice. CD31 whole-mount stained tracheas were quantitated for microsphere extravasation (<b>B</b>). Adenoviral delivery of Ang-2 in Ang-2^−/− mice rescued the phenotype of Ang-2^−/− mice and facilitated the histamine response. Moreover, delivery of Ad-Ang-2 in WT mice enhanced the response to histamine. Values are expressed as mean ± SD (n = 6, saline n = 3). **p<0.01 compared to Ad-LacZ and histamine.</p